Cumulase can replace animal-derived hyaluronidases used to prepare oocytes for certain In-Vitro Fertilization procedures and cryopreservation. The purity of Cumulase is up to 100-fold higher than the currently used animal preparations. It may reduce, if not eliminate, the risk of damage or trauma to the oocytes, and eliminates the risk of animal pathogen contamination, thereby providing a safer, purer, and more reliable alternative to slaughterhouse-derived enzymes.